Valorizing hazardous lead glass sludge and alumina flakes filling waste for the synthesis of geopolymer building bricks.

Geopolymer bricks from lead glass sludge (LGS) and alumina flakes filling (AFF) waste were synthesized in the present work. AFF waste was chemically treated to prepare sodium aluminate (NaAlO2) powder. Silicate source (untreated LGS and thermally treated one at 600 °C (LGS600)) and sodium oxide (Na2O) concentration (as NaAlO2) were the compositional parameters, which affected the physical and mechanical properties (compressive strength, water absorption, and bulk density) of the prepared bricks. High organic matter content inside LGS caused a retardation effect on the geopolymerization process, resulting in the formation of hardened bricks with modest 90-day compressive strengths (2.13 to 4.4 MPa). Using LGS600 enhanced the mechanical properties of the fabricated bricks, achieving a maximum 90-day compressive strength of 22.35 MPa at 3 wt.% Na2O. Sodium aluminosilicate hydrate was the main activation product inside all samples, as confirmed by X-ray diffraction and thermal analyses. Acetic acid leaching test also proved that all LGS600-NaAlO2 mixtures represented Pb concentrations in leachates lower than the permissible level of characteristic leaching procedures, indicating the mitigation of environmental problems caused by these wastes.

Alkaline treatment combined with enzymatic hydrolysis for efficient cellulose nanofibrils production.

Cellulose nanofibrils were efficiently produced from eucalyptus fibers using a combined NaOH and enzymatic treatment followed by a pilot scale grinding process. The structural changes of fibers were assessed after NaOH treatments at 5, 10 and 15 wt% concentrations. A progressive shift from a cellulose I to a cellulose II crystalline structure was observed with X-ray diffraction (XRD) and nuclear magnetic resonance (NMR). The further enzymatic hydrolysis was improved for the NaOH treated samples. The increase of crystallinity indices due to enzymatic hydrolysis was of + 4.7 %, + 3.5 %, and +10.3 % for samples treated with NaOH 5, 10 and 15 wt% respectively, and DP values were drastically reduced to 340, 190 and 166 respectively. A morphological analysis underlined an optimum with the combination of NaOH 10 wt% and enzymatic hydrolysis. This treatment followed by the grinding process resulted in CNF with a rigid structure, with diameters ranging from 10 to 20 nm and lengths between 150 and 350 nm. A multi-scale analysis enabled to study the impact of this combined treatment on CNF properties and energy consumption. A decrease in mechanical properties of nanopapers was observed for the combined treatment and NaOH treatment alone compared to enzymatic hydrolysis alone, with Young's modulus of 8.94, 4.84 and 11.21 GPa respectively. However, optical properties were improved, with transmittance values of 42.2, 15.4 and 7.1 % respectively. This new pretreatment can therefore lead to CNF with tunable properties depending on the application, with possible industrialization thanks to the reduction of energy needs.

Effects of Desilication in NaOH/Piperidine Medium and Phosphorus Modification on the Catalytic Activity of HZSM-5 Catalyst in Methanol to Propylene Conversion.

BACKGROUND: Propylene is one of the main petrochemical building blocks applied as a feedstock for various chemical and polymer intermediates. The methanol-to-propylene (MTP) processes are reliable options for propylene production from non-petroleum resources. The highsilica ZSM-5 zeolite is found to be a reliable candidate for the methanol to propylene catalysis. OBJECTIVE: In this study, the mesoporosity was first introduced into a high silica ZSM-5 zeolite via an alkaline treatment by NaOH solution with piperidine to decrease the diffusion limitation, and then the structure of zeolite was stabilized by phosphorus modification to improve the acidic properties and to enhance the catalyst stability. METHODS: High-silica H-ZSM-5 catalysts (Si/Al = 200) were successfully prepared through microwave-assisted hydrothermal technique in the presence of tetrapropyl ammonium hydroxide (TPAOH) structure-directing agent. The mesoporosity was efficiently introduced into the ZSM-5 crystals via desilication derived from alkaline NaOH/piperidine solution. Then, the acidity of the desilicated ZSM-5 samples was improved using phosphorus modification. The catalysts were subjected to XRD, ICP-OES, FE-SEM, BET, TGA, FT-IR and NH3-TPD analysis. RESULTS: The catalytic performance of the prepared catalysts in the methanol to propylene (MTP) reaction was examined in a fixed-bed reactor at 475 °C, atmospheric pressure and methanol WHSV of 0.9 h-1. The results showed that the alkaline treatment in NaOH/piperidine solution created uniform mesoporosity with no severe damage in the crystal structure. Similarly, phosphorus modification developed the acidic features and led to the optimal catalytic efficiency in terms of the maximum propylene selectivity (49.16%) and P/E ratio (5.97) as well as the catalyst lifetime. CONCLUSION: The results showed an excellent catalytic activity in terms of 99.21% methanol conversion, good propylene selectivity up to 49.16%, a high ratio of P/E of 5.97 and a low selectivity to C5 + hydrocarbons of 11.57% for ZS-D-PI-P sample.

Valorization of orange bagasse through one-step physical and chemical combined processes to obtain a cellulose-rich material.

BACKGROUND: Orange bagasse (OB) is an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose. The present study aimed to investigate different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB and to characterize the materials obtained according to their composition, morphology, crystallinity and thermal stability. RESULTS: The processing yields ranged from 140 to 820 g kg-1 , with a recovery of 720-1000 g kg-1 of the original cellulose. Treatments promoted morphological changes in the fiber structure, resulting in materials with higher porosity, indicating partial removal of the noncellulosic fractions. The use of combined chemical treatments (NaOH and peracetic acid) with autoclaving was more efficient for obtaining samples with the highest cellulose contents. CONCLUSION: Therefore, ACSH (processed by autoclaving with NaOH) was the most effective one-step treatment, resulting in 71.1% cellulose, 0% hemicellulose and 19.0% lignin, with a crystallinity index of 42%. The one-step treatments were able to obtain materials with higher cellulose contents and yields, reducing reaction times and the quantity of chemical reagents employed in the overall processes compared to multistep conventional processes. © 2020 Society of Chemical Industry.

Superfine pulverisation pretreatment to enhance crystallinity of cellulose from Lycium barbarum L. leaves.

Superfine pulverisation (SFP) pretreatment of Lycium barbarum L. leaves was performed to obtain highly crystalline cellulose. Compared with other common pulverisation methods, SFP enhanced cellulosic crystallinity by 18.3 % and 8.4 %, with and without post-acid treatments, respectively. XRD and solid-state NMR analyses showed that SFP facilitated the exposure of amorphous substances (i.e., hemicellulose and lignin) to NaOH and H2O2. Large amounts of silicon (5.5 %) and aluminium (2.1 %) were found to incorporate into the crystalline regions of SFP-produced cellulose. Further FTIR and thermogravimetric analyses revealed that SFP-produced cellulose contained large amounts of hydroxyl groups, affecting the cellulosic crystallinity and thermal stability. These findings demonstrate the potential for SFP to serve as a green technology for production of highly crystalline and mineral-rich cellulose.

Deconstruction of banana peel for carbohydrate fractionation.

The deconstruction of banana peel for carbohydrate recovery was performed by sequential treatment (acid, alkaline, and enzymatic). The pretreatment with citric acid promoted the extraction of pectin, resulting in a yield of 8%. In addition, xylose and XOS, 348.5 and 17.3 mg/g xylan, respectively, were also quantified in acidic liquor as a result of partial depolymerization of hemicellulose. The spent solid was pretreated with alkaline solution (NaOH or KOH) for delignification and release of residual carbohydrates from the hemicellulose. The yields of xylose and arabinose (225.2 and 174.0 mg/g hemicellulose) were approximately 40% higher in the pretreatment with KOH, while pretreatment with NaOH promoted higher delignification (67%), XOS yield (32.6 mg/g xylan), and preservation of cellulosic fraction. Finally, the spent alkaline solid, rich in cellulose (76%), was treated enzymatically to release glucose, reaching the final concentration of 28.2 g/L. The mass balance showed that through sequential treatment, 9.9 g of xylose, 0.5 g of XOS, and 8.2 g of glucose were obtained from 100 g of raw banana peels, representing 65.8% and 46.5% conversion of hemicellulose and cellulose, respectively. The study of the fractionation of carbohydrates in banana peel proved to be a useful tool for valorization, mainly of the hemicellulose fraction for the production of XOS and xylose with high value applications in the food industry.

Photoluminescence as a Complementary Tool for UV-VIS Spectroscopy to Highlight the Photodegradation of Drugs: A Case Study on Melatonin.

In this work, a complementary ultraviolet-visible (UV-VIS) spectroscopy and photoluminescence (PL) study on melatonin (MEL) hydrolysis in the presence of alkaline aqueous solutions and the photodegradation of MEL is reported. The UV-VIS spectrum of MEL is characterized by an absorption band with a peak at 278 nm. This peak shifts to 272 nm simultaneously with an increase in the band absorbance at 329 nm in the presence of an NaOH solution. The isosbestic point localized at 308 nm indicates the generation of some chemical compounds in addition to MEL and NaOH. The MEL PL spectrum is characterized by a band at 365 nm. There is a gradual decrease in the MEL PL intensity as the alkaline solution concentration added at the drug solution is increased. In the case of the MEL samples interacting with an alkaline solution, a new photoluminescence excitation (PLE) band at 335 nm appears when the exposure time to UV light reaches 310 min. A down-shift in the MEL PLE band, from 321 to 311 nm, as a consequence of the presence of excipients, is also shown. These changes are explained in reference to the MEL hydrolytic products.

New light on plant ash glass found in Africa: Evidence for Indian Ocean Silk Road trade using major, minor, trace element and lead isotope analysis of glass from the 15th-16th century AD from Malindi and Mambrui, Kenya.

Seventeen glass vessels and twenty glass beads recovered from the excavations at the ancient city of Malindi and the archaeological site of Mambrui in Kenya, east Africa were analysed using electron probe microanalysis (EPMA) and laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). The results show that all of the glass samples are soda-lime-silica glass. They belong to the high alumina -plant ash glass type, characterised by high alumina and relatively low calcium contents, widely distributed in eastern (10th- 16th centuries AD) and southern Africa (13th - 15th centuries AD), Central Asia (9th- 14th centuries AD) and southeast Asia (12th- 13th centuries AD), made with plant ashes and sands. This is an understudied glass type for which previous research has indicated there were three types. When compared with published research on such glasses using Zr, Ti, Ba, Cr, La, Li, Cs, Na2O, MgO and CaO we have identified at least four different compositional groups of v-Na-Al glass: Types A, B, C and D. By comparing the results with contemporary v-Na-Al glass vessels and beads from Central Asia, Africa, and southeast Asia we show that most of the Malindi and Mambrui glass share similar characteristics to the compositions of Mapungubwe Oblate and some of the Madagascar glass beads from southern Africa. They belong to Type A v-Na-Al glass which is characterised by an elevated level of Ti and Ba and a relatively high ratios of Cr/La, relatively low Zr concentrations and low ratios of Zr/Ti. Differences in Zr, Li, MgO and Na2O concentrations in Type A glass indicates that there are subgroups which might derive from different glass workshop(s) specialising in Type A v-Na-Al glass production. Comparison with the chemical compositions of glass from Ghazni, Afghanistan and Termez, Uzbekistan, and by using lead isotope analysis, we suggest v-Na-Al glass was manufactured in Central Asia and possibly worked into vessels and beads there.

Combination of dispersive solid phase extraction with solidification organic drop-dispersive liquid-liquid microextraction based on deep eutectic solvent for extraction of organophosphorous pesticides from edible oil samples.

A dispersive solid phase extraction method was combined with deep eutectic solvent-based solidification of floating organic drop-dispersive liquid-liquid microextraction and used for the extraction/preconcentration of some organophosphorus pesticides residues from edible oil samples. The extracted analytes were quantified with gas chromatography-nitrogen phosphorous detector. In this procedure, the sample lipids are saponified with a sodium hydroxide solution and then the analytes are adsorbed onto a primary secondary amine sorbent. After that the analytes are desorbed with acetone as an elution/dispersive solvent and mixed with choline chloride: 3,3-dimethyl butyric acid deep eutectic solvent and the mixture is rapidly dispersed into deionized water. Then, the obtained cloudy solution is centrifuged and placed into an ice bath. The extraction solvent is solidified on the top of the solution. Finally, it is removed and dissolved in acetonitrile, and 1 µL of the solution is injected into the separation system. Validation of the method showed that limits of detection and quantification were in the ranges of 0.06-0.24 and 0.20-0.56 ng mL-1, respectively. Enrichment factors and extraction recoveries of the analytes ranged from 170-192 and 68-77%, respectively. The method had an acceptable precision with relative standard deviations less than ≤9.2% for intra- (n=6) and inter-day (n=6) precisions at four concentrations (3, 10, 50, and 250 ng mL-1, each analyte). Finally the method was used for determination of the analytes in five edible oil samples.

Assessing efficacy of N-Acetyl-l-Cysteine-Sodium Hydroxide on bacterial viability and enhanced recovery of Mycobacterium avium subsp. paratuberculosis from bovine colostrum.

The standard procedure for the improved cultural recovery of viable Mycobacterium spp. from diverse samples mainly depends on reducing the viability of background microbiota using different chemical compounds. This study was designed to i) evaluate the efficacy and comparison between N-Acetyl-l-Cysteine-Sodium hydroxide (NALC-2% NaOH) and hexadecylpyridinium chloride (0.75% HPC) treatment and exposure time on reducing the viability of undesirable microorganisms with minimal impact on colostrum consistency; and ii) assess the impact of NALC-2% NaOH on improved and enhanced recovery of Mycobacterium avium subsp. paratuberculosis (MAP) in spiked postpartum colostrum samples and consistency of colostrum. A total of 40 samples, each treated with NALC-2% NaOH for 15 min or 0.75% HPC for 5 h, were investigated for total mesophilic aerobic bacteria (MAB) and enterobacteria (EB) (CFU mL-1). The results showed that treatment of colostrum samples with NALC-2% NaOH completely eliminated EB and significantly reduced MAB (3.6 log10 CFU mL-1). Conversely, samples treated with 0.75% HPC produced a complex mixture following interaction with the colostrum protein and showed non-significant and variable results. In addition, the spiked colostrum treated with NALC-2% NaOH for 15 min revealed recovery of viable MAP cells with a minimum limit of detection of 1.36 log10 CFU 10 mL-1 where no change in the consistency of colostrum was observed. In conclusion, 15-min NALC-2% NaOH treatment of colostrum may significantly reduce the viability of undesirable microorganisms and help to enhance the efficient recovery of MAP without impacting the consistency of high quality postpartum colostrum. This rapid procedure is suitable for efficient recovery and early detection of MAP as well as preventing its transmission to neonates and young calves in MAP infected herds.

The structure-property relationships of acid- and alkali-extracted grapefruit peel pectins.

Structure and properties of pectin can be affected by extraction methods. In this study, grapefruit peel pectins extracted by HCl (at pH 1 [P1], 2 [P2], and 3 [P3]) and NaOH (at pH 9 [P9], 10 [P10], and 11 [P11]) were prepared and characterized. Atomic force microscopy (AFM) provided direct evidence of complex nano-structural patterns of pectins and revealed cross-linked networks of P10 and P11. Small-angle X-ray scattering (SAXS) demonstrated that P1, P2, and P3 possessed a relatively extended conformation, whereas P9, P10, and P11 displayed a three-dimensional structure and folded conformation. The compact and extended conformations of P3 contributed to its high viscosity in solution and the stability of the formed emulsion (75%). Porous surface and larger three-dimensional nanostructure (Dmax: 23 nm) of P10 facilitated its ion-binding capacity. Our results provide valuable insight into relationship between extraction methods and structure-properties of pectin, facilitating design of functional pectins.

Hydroxypropylation of cross-linked sesbania gum, characterization and properties.

Sesbania gum (SG) was successfully modified by combining cross-linking with hydroxypropylation in solvent of ethanol. The hydroxypropylation technology parameters were optimized by the orthogonal test. The results indicated that both cross-linking and hydroxypropylation could improve the freeze-thaw stability, acid and alkali resistance of SG. After the cross-linking and hydroxypropylation of SG, the retrogradation was evidently strengthened. The surfaces of SG derivatives such as cross-linked sesbania gum (CLSG) and hydroxypropyl cross-linked sesbania gum (HCLSG) particles also became very rough, compared with SG. The sequence combination of first cross-linking and then hydroxypropylation was helpful to the follow-up hydroxypropylation of SG. The cross-linking and hydroxypropylation resulted in an obvious reduction in the melting enthalpy of SG, which belonged to a semicrystalline polymer. The cross-linking and hydroxypropylation influenced the thermal properties, and destroyed the crystalline regions of SG. According to SEM, the hydroxypropylation has greater contribution to the destruction than cross-linking. Both cross-linking and hydroxypropylation gave rise to the expansion of SG grains, but the swelling manner was different each other.

Biorefining and the Functional Properties of Proteins from Lipid and Pigment Extract Residue of Chlorella pyrenoidosa.

Microalgae are considered as excellent candidates for bioactive compounds, yet microalgal residues remaining after the extraction of one or two compounds are usually discarded, which is not economical. This study demonstrates the alkaline extraction of proteins from Chlorella pyrenoidosa residue after lipid and pigment extractions, and their functional properties. Single-factor experiments and response surface methodology were used to obtain the optimal conditions for protein extraction. Based on our results, a maximum protein yield of 722.70 mg/g, was obtained under the following extraction conditions: sodium hydroxide concentration 7.90%, extraction temperature 70.00 °C, extraction time 34.80 min, and microalgal residue concentration 8.20 mg/mL. The molecular weight of microalgal residue protein isolate (MRPI) was mainly distributed at the regions of 0.18-0.50 kDa, 0.50-1.50 kDa, and 1.50-5.00 kDa. The essential amino acid content was greater than the values recommended by FAO/WHO standards; a high essential amino acid index value (1.49) was another good indication that MRPI is suitable for human consumption. Moreover, MRPI exhibited excellent emulsifying properties and antioxidant activity, which suggests it may be useful as an emulsifying agent and antioxidant. These findings could improve the extraction methods of functional protein from microalgal residue and add value to microalgae-based bioactive compound production processes.

Characterization of cellulose fibers in Thespesia populnea barks: Influence of alkali treatment.

Natural fibers are emerging as best alternatives for synthetic materials in selective applications. These fibers may not have the required properties in its raw form and hence needs some alterations in its characteristics. Likely, this article reports enhancement in surface and structural properties of Thespesia populnea bark fiber treated with NaOH under various concentration and soaking period. Fibers treated with 5% NaOH for 60 min yields noteworthy mechanical strength (678.41 ± 48.91 MPa) owing to its relatively high cellulose fraction (76.42%). Fourier transform infrared spectra endorses the removal of non-cellulosic compounds and X-ray diffraction studies reveals 13.6% growth in the size of cellulose crystals on optimally treated fibers. Weibull distribution model statistically interprets the reliability of acquired tensile test results. Finally, microscopic examinations with scanning electron microscopy and atomic force microscopy explore that fiber surface turns rough after alkali treatment and makes it appropriate for reinforcement in polymer matrices.

Chemically crosslinked hydrogel and its driving force towards superabsorbent behaviour.

Dissolved oil palm empty fruit bunch (EFB) cellulose in NaOH/urea solvent was mixed with sodium carboxymethylcellulose (NaCMC) to form a green regenerated superabsorbent hydrogel. The effect of concentration of epichlorohydrin (ECH) as the crosslinker on the formation, physical, and chemical properties of hydrogel was studied. Rapid formation and higher gel content of hydrogel were observed at 10% concentration of ECH. The superabsorbent hydrogel was successfully fabricated in this study with the swelling ability >100,000%. Hydrogel with higher concentration of ECH showed opposite trend by having higher superabsorbent property than that of lower concentration. The covalent bond of COC was observed with Attenuated total reflectance fourier transform infrared (ATR-FT-IR) spectroscopy to confirm the occurrence of crosslinking. The physical and chemical properties of hydrogel were affected by swelling phenomenon. Hydrogel with higher degree of swelling exhibited lower moisture retention and higher transparency. Moreover, the weight of the superabsorbent hydrogel increased with the decrement of pH value of external media (distilled water). This study provided substantial information on the effect of different percentage of ECH as crosslinker on hydrogel basic properties. Furthermore, this study affords correlation of many essential driving forces that affected hydrogel superabsorbent property.

Efficacy of activated persulfate in inactivating Escherichia coli O157:H7 and Listeria monocytogenes.

Concerns have been on the rise regarding the use of chlorine-based sanitizers for fresh produce sanitation due to the production of toxic disinfection by-products (DBPs). This study was undertaken to evaluate the efficacy of activated persulfate in inactivating Escherichia coli O157:H7 and Listeria monocytogenes in pure culture. The objectives were to study the effect of persulfate to activator ratios and determine the major contributing radical in pathogen inactivation. A five-strain cocktail of each pathogen was treated with sodium persulfate activated by ferrous sulfate or sodium hydroxide for 60 s or 120 s. Non-selective agars supplemented with sodium pyruvate were used for pathogen enumeration. The steady-state concentrations of free radicals were quantified using HPLC-DAD. Radical scavengers (tert-butanol, isopropanol, and benzoquinone) were used to determine the major contributing radical in pathogen inactivation. The results showed more than 7 log CFU/mL reductions can be achieved in 120 s for both pathogens at appropriate activation conditions. For ferrous activation, the persulfate to ferrous ratio played an important role in the overall inactivation efficacy. The maximum pathogen reduction (7.77 log CFU/mL for E. coli O157:H7 and 7.25 log CFU/mL for L. monocytogenes) was achieved at persulfate to ferrous molar ratio of 1:0.33 when the initial persulfate concentration was set at 40 mmol/L. Further increase or decrease of ferrous ratio always leads to lower pathogen reductions. For alkaline activation, the inactivation efficacy increased with more initial sodium hydroxide. The maximum reduction was achieved at 40 mmol/L persulfate with 30 mmol/L sodium hydroxide for E. coli O157:H7 (6.21 log CFU/mL reduction) and at 500 mmol/L persulfate with 350 mmol/L sodium hydroxide for L. monocytogenes (8.64 log CFU/mL reduction). Also, persulfate activated by sodium hydroxide always achieved significantly (P < 0.05) higher microbial reductions than sodium hydroxide or persulfate alone. L. monocytogenes was generally more resistant against the activated persulfate treatment compared with E. coli O157:H7, which might be due to the different cell envelop structures between Gram-positive and Gram-negative bacteria. Hydroxyl radical was demonstrated to be the major radical to inactivate both pathogens in ferrous activation while superoxide radical was demonstrated to be the major radical to inactivate both pathogens in alkaline activation.

CO2-assisted phosphorus extraction from poultry litter and selective recovery of struvite and potassium struvite.

Phosphorus recovery from industrialized poultry operations is necessary to ensure sustainable waste management and resource consumption. To realize these goals, an innovative, two-stage process chemistry has been developed to extract nutrients from poultry litter and recover value-added products. Over 75% phosphorus extraction was achieved by bubbling carbon dioxide into poultry litter slurries and adding strong acid to reach pH 4.5-5.5. After separating the nutrient-deficient poultry litter solids and the nutrient-rich liquid, the extract pH was increased through aeration and strong base addition. Over 95% of the extracted phosphorus was recovered as solid precipitate at pH 8.5-9.0. High-purity struvite and potassium struvite products were selectively recovered through pH control, introduction of a calcium-complexing agent, and addition of magnesium chloride. The nitrogen-to-phosphorus-to-potassium (NPK) ratio of the recovered solids was controlled through aeration and pH adjustment. Precipitation at pH 8.5-9.0 and 10.5-11.0 resulted in NPK ratios of 2.0:1.0:0.1 and 0.9:1.0:0.2, respectively. The process effluent was effectively recycled as makeup water for the subsequent batch of poultry litter, thereby decreasing water consumption and increasing overall nutrient recovery. Sequencing batch operation yielded greater than 70% phosphorus recovery within a 45-min process, demonstrating the potential for this technology to alleviate nutrient pollution in agricultural settings and generate an alternative supply of phosphorus fertilizers.

Antimicrobial Activity of the Phenolic Compounds of Prunus mume against Enterobacteria.

Mume fruit, the Japanese apricot (Prunus mume SIEB. et ZUCC.), is popular in Japan and is mostly consumed in the pickled form called umeboshi. This fruit is known to have anti-microbial properties, but the principal constituents responsible for the antimicrobial properties have not yet been elucidated. We investigated the antimicrobial activities of the phenolic compounds in P. mume against enterobacteria. In this study, growth inhibitory activities were measured as an index of the antibacterial activities. The phenolic compounds were prepared from a byproduct of umeboshi called umesu or umezu (often translated as "mume vinegar"). Umesu or umezu phenolics (UP) contain approximately 20% phenolic compounds with p-coumaric acid as a standard and do not contain citric acid. We observed the inhibitory effects of UP against the growth of some enterobacteria, at a relatively high concentration (1250-5000 µg/mL). Alkali hydrolysates of UP (AHUP) exhibited similar antibacterial activities, but at much lower concentrations of 37.5-300 µg/mL. Since AHUP comprises hydroxycinnamic acids such as caffeic acid, p-coumaric acid, and ferulic acid, the antibacterial activities of each of these acids were examined. Our study shows that the phenolic compounds in P. mume other than citric acid contribute to its antimicrobial activity against enterobacteria in the digestive tract.

Internal phosphorus load in a Mexican reservoir through sediment speciation analysis.

Since the sequential extraction of phosphorus (P) in sediment makes it possible to determine the P potentially available for release, in this paper, we evaluate the fractions of P in sediment profiles from Valle de Bravo reservoir, a eutrophic lake in central Mexico to determine the contributions of each fraction to the internal P load (IPL). The P fractionation scheme employs sequential extractions of sediment with O2-free water (MilliQ), bicarbonate-dithionite (BD), sodium hydroxide (NaOH), hydrochloric acid (HCl), and potassium persulfate (K2S2O8-) to obtain five P fractions. A monitoring of redox potential (Eh), pH, and total phosphorus (TP) in the bottom water of the reservoir indicated variations of these parameters during the year, observing that as Eh decreased, the P concentration increased, it was also observed that when increasing pH, P concentration also increased. Analyzing the behavior of fractions of P in sediment profiles, we found that the dominant fractions are those bound to iron and aluminum oxides, corresponding to approximately 50% of total P since P concentrations of these fractions were twice as high in the top 5 cm of the sediment profiles and decreased with increasing depth. Considering the variations of Eh and pH in the bottom water of the reservoir and that these parameters are factors that control the release of P with the fractions of P bound to Fe/Mn and Al/Fe oxides, we concluded that these fractions contribute most to P potentially available for release in the reservoir, representing a possible IPL of 23.5 ± 1.4 t/year.

Phosphorus in recycling fertilizers - analytical challenges.

The importance of secondary raw materials for phosphorus (P) fertilizer production is expected to increase in the future due to resource depletion, supply risks, and heavy metal contamination of fossil phosphate resources. Municipal wastewater is a promising source for P recovery. In Germany for instance, it contains almost 50% of the total amount of P that is currently applied as mineral fertilizer. Several procedures have been developed to recover and re-use P resulting in a growing number of recycling fertilizers that are currently not regulated in terms of fertilizer efficiency. We tested various materials and matrices for their total P content, solubility of P in neutral ammonium citrate (Pnac) and water, and performed robustness tests to check if existing analytical methods are suitable for those new materials. Digestion with inverse aqua regia was best suited to determine the total P content. Pnac sample preparation and analyses were feasible for all matrices. However, we found significant time and temperature dependencies, especially for materials containing organic matter. Furthermore, several materials didn't reach equilibrium during the extractions. Thus, strict compliance of the test conditions is strongly recommended to achieve comparable results.